Ultraviolet spectrophotometric method for the determination of the purity of substances - Determination of trace benzene in ethanol - Database & Sql Blog Articles

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Determination of Purity of Substance by Ultraviolet Spectrophotometry: Detection of Trace Benzene in Ethanol

Keywords: ultraviolet spectrophotometry; trace benzene in ethanol; aesthetic analyzer; UV-1100

Width="1412" height="457" src="http://i.bosscdn.com/blog/nophoto.gif" /></p> <p>The main objective of this experiment is to understand and apply the principles of ultraviolet (UV) spectrophotometry for checking the purity of substances. Specifically, we will learn how to detect trace amounts of benzene in ethanol using a UV-1100 spectrophotometer. This technique is widely used in analytical chemistry due to its sensitivity and ease of use.</p> <p>Ultraviolet absorption spectroscopy is a powerful tool in organic analysis. It works by measuring the absorption of light at specific wavelengths, which helps identify the presence of certain compounds. Benzene, for example, has a distinct absorption band between 230 nm and 280 nm, while ethanol and cyclohexane do not show significant absorption in this range. By comparing the absorbance values, we can determine if there are any impurities such as benzene in the sample.</p> <p><strong>Equipment and Materials:</strong> UV-1100 spectrophotometer, quartz cuvettes, 10 mL volumetric flask, 5 μL micro-injector, absolute ethanol, and pure benzene.</p> <p><strong>Procedure:</strong></p> <ol> <li>Prepare a solution by adding 2 μL of pure benzene into a 10 mL volumetric flask and diluting it with absolute ethanol.</li> <li>Measure the absorbance of absolute ethanol at various wavelengths (from 230 nm to 280 nm).</li> <li>Repeat the same process for the benzene solution to compare the absorption spectra.</li> </ol> <p><strong>Data Analysis:</strong> Plot an absorbance vs. wavelength graph to observe any differences between the two samples. If benzene is present, a clear peak should appear in the 230–280 nm range.</p> <p><strong>Notes:</strong></p> <ul> <li>Handle quartz cuvettes carefully—only touch the top surface to avoid scratches.</li> <li>Make sure the cuvette is clean and dry before placing it in the spectrophotometer.</li> <li>If negative readings occur during the experiment, consult your instructor immediately.</li> <li>After the experiment, do not wash the cuvette with water. Let it air dry or rinse with a small amount of absolute ethanol.</li> </ul> <p>This experiment provides hands-on experience in using advanced analytical techniques and reinforces the importance of precision and accuracy in chemical analysis.</p> </dt> </div> <div class=

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